Technical Data
F0019-98J
FBG4 (F-box 4)
Description:
F-box proteins, a critical component of the evolutionary conserved ubiquitin-protein ligase
complex SCF (Skp1/Cdc53-Cullin1/F-box), recruit substrates for ubiquitination and consequent degradation through their specific protein-protein interaction domains. Novel human F-box proteins named FBG1, FBG2, FBG3, FBG4 and FBG5 constitute a third subfamily of mammalian F-box proteins. FBG genes are expressed in a limited number of human tissues including kidney, liver, brain and muscle tissues.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500
Optimal dilutions to be determined by the researcher.

Positive control: Recombinant human full-length FBG4.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to aa70-85 (LQLARDRSAEGRALYA) of human FBG4 conjugated to KLH at the N-terminus.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity:
Recognizes human FBG4 (aa70-85). Sequence homology: mouse, rat (100%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ilyin, G.P., et al., (2002), “A new subfamily of structurally related human F-box proteins”, Gene, 296(1-2): 11-20.