Technical Data
FASN (Fatty Acid Synthase)
FAS (Fatty Acid Synthase) is a central enzyme in de novo lipogenesis (triglyceride production). It is a target for SREBP and is upregulated by LXR activation. Fatty liver is often the result of FAS activation. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation.

Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 A band should be seen at ~272kD on SDS-PAGE. May see 1 or 2 minor cross-reacting lower MW bands in liver tissue.
Immunoprecipitation: 1:100
Immunohistochemistry: Paraffin
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse liver lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceMouseRabbit
As reported
Internal synthetic peptide corresponding to the N-terminus of mouse FASN (KLH). Cellular Localization: Cytoplasmic
Supplied as a liquid, 0.05% sodium azide.
Recognizes mouse FASN. Species Crossreactivity: Human, porcine, rat, monkey, chicken, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dridi S, et al. Am J Physiol Regul Integr Comp Physiol. 2006, 291(1):R138-47. 2. Gao, H., et al. Long-term administration of estradiol decreases expression of hepatic lipogenic genes improves insulin sensitivity in ob/ob mice: a possible mechanism is through direct regulation of Stat3. Mol. Endocrinol. 2006. 3. Heemers, H.V., et al. Cancer Res. 2007 67: 3422-3430. 4. Vavere, A. L., et al. J Nucl Med. 2008; 49: 327-334. 5. Bryzgalova G, et al. Am J Physiol Endocrinol Metab. 2008, 295(4):E904-12. 6. Gao H, et al. Mol Endocrinol. 2006; 20(6):1287-99. 7. Basciano H, et al. Am J Physiol Gastrointest Liver Physiol 2009, 297(2):G323-332. 8. Basciano H, et al. Am J Physiol Endocrinol Metab 2009:90764.92008.