Technical Data
F0024-45A
FBXO44 (F-box Only Protein 44, F-box Protein FBX30, F-box/G-domain Protein 3, FBG3, FBX30, FBX44, FBX6A, FBXO6A)
Description:
F-box proteins, a critical component of the evolutionary conserved ubiquitin-protein ligase complex SCF (Skp1/Cdc53-Cullin1/F-box), recruit substrates for ubiquitination and consequent degradation through their specific protein-protein interaction domains. Novel human F-box proteins named FBG1, FBG2, FBG3, FBG4 and FBG5 constitute a third subfamily of mammalian F-box proteins. FBG genes are expressed in a limited number of human tissues including kidney, liver, brain and muscle tissues.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000
Western Blot: 2-6ug/ml, observed in human brain (cerebellum) lysate on ~25kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-AALTPPEPPSAEP, from human FBXO44, at C-terminal (NP_904319.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human FBXO44.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ilyin G, Serandour A, Pigeon C, Rialland M, Glaise D, Guguen-Guillouzo C. A new subfamily of structurally related human F-box proteins. Gene. 2002 Aug 21;296(1-2):11.