Technical Data
FBXW2 (F-box and WD Repeat Domain Containing 2, F-box and WD40 Domain Protein 2, FBW 2, FBW2)
F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. A large family of mammalian F-box proteins has recently been identified and classified into three groups based on the presence of either the WD-40 repeats, the leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. The FBXW2 gene product, the second identified member of the F-box gene family, contains multiple WD-40 repeats.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide: KRGSSFLAGEHPG representing the C-Terminus of the human protein (residues 410-422), according to NP_036296.
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
Species Crossreactivity: Crossreacts with Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Winston JT et al. A family of mammalian F-box proteins. Curr Biol 9:1180-2 (1999).