Technical Data
F0045-01
FEM1A (Protein Fem-1 Homolog A, FEM1a, FEM1-alpha, Prostaglandin E Receptor 4-associated Protein, EPRAP)
Description:
Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. May participate in antiinflammatory signaling via its interaction with PTGER4.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000
Western Blot: 1-3ug/ml, observed in human heart lysates on ~73.6kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-GFIPEDLEAFIELH, from human FEM1A, at C-terminal (NP_061178.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human FEM1A. Species sequence homology: Bovine and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Minami M, Shimizu K, Okamoto Y, Folco E, Ilasaca ML, Feinberg MW, Aikawa M, Libby P. Prostaglandin E receptor type 4-associated protein interacts directly with NF-kappaB1 and attenuates macrophage activation. J Biol Chem. 2008 Apr 11;283(15):9692-703.