Technical Data
F0046
Fen1 (FEN-1, Flap Structure-specific Endonuclease 1, MF1, Maturation Factor-1, RAD2, DNase IV)
Description:
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42kD protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

Application(s):
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-5mg/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Fusion between BALB/c splenocytes and mouse myeloma (NS-1 cells).

Positive Control:
MCF-7 cells, recombinant fusion protein.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k4i261Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant human FEN-1 protein encoding amino acids 1-380 purified from baculovirus-infected Sf-9 cells.
Purity:
Purified from hybridoma cell culture supernatant by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.
Form
Supplied as a liquid in 10mM PBS (pH 7.4).
Specificity:
Reacts with human FEN-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
General:
1.Kolodner, R.D. and Marsischky, G.T. (1999) Eukaryotic DNA mismatch repair. Curr Opin Genet Dev 9:89-96.
2.Rumbaugh, J.A., Henricksen, L.A., DeMott, M.S. and Bambara, R.A. (1999) Cleavage of substrates with mismatched nucleotides by flap endonuclease-1. Implications for mammalian Okazaki fragment processing. J Biol Chem 274:14602-8
3.Kim, K., Biade, S. and Matsumoto,Y. (1998) Involvement of flap endonuclease 1 in base excision DNA repair. J Biol Chem 273:8842-8.
4.Warbrick, E., Coates, P.J. and Hall, P.A. (1998) Fen1 expression: a novel marker for cell proliferation. J Pathology 186:319-24.
5.Hiraoka, L., Harrington, J., Gerhard, D.S., Lieber, M.R. and Hsieh, C.L. (1995) Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human. Genomics 25:220-5.



Specific:
1. Qiu, J., et al. Cell Cycle-dependent and DNA Damage-inducible Nuclear Localization of FEN-1 Nuclease Is Consistent with Its Dual Functions in DNA Replication and Repair*. The Journal of Biological Chemistry. 276(7): 4901-4908, 2001.