		Technical Data
F0046-01	FEN1 (Flap Endonuclease 1, FEN-1, DNase IV, Flap Structure-specific Endonuclease 1, Maturation Factor 1, MF1, hFEN-1, RAD2)

Description:
FEN-1 is a 42kD protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	4E7	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant corresponding to aa1-380 from human FEN1 expressed in E. coli.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS.

Specificity:

Recognizes human FEN1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.