Technical Data
F0047-02
Fen1 (FEN-1, Flap Structure-specific Endonuclease 1, MF1, Maturation Factor-1, RAD2, DNase IV)
Description:
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42kD protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
A synthetic peptide, which represented a portion of human Flap Structure-specific Endonuclease 1 encoded within exon 2 (LocusLink ID 2237). The peptide is derived from a portion of the latter half of the N-domain as defined I Swiss-Prot entry P39748.
Purity:
Purified by immunoaffinity chromatography.
Form
As reported
Specificity:
Specific for human FEN-1 protein. Other species have not been tested. Reactivity with mouse FEN-1 is likely owing to complete identity between ortholog and the peptide used as the immunogen and Xenopus FEN-1 has 1 mismatch with the peptide used as the immunogen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.