Technical Data
Fen1 (FEN-1, Flap Structure-specific Endonuclease 1, MF1, Maturation Factor-1, RAD2, DNase IV)
FEN-1 (Flap EndoNuclease and Five prime Exonuclease) is a 42kDa protein that functions as an endonuclease and cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair, and is required in cell-free DNA replication systems.

Positive Control: HeLa, Raji and 3T3 cell lysates.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined
Recombinant human FEN-1 protein purified from E. coli.
Whole antisera
As reported
Recognizes human FEN-1. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hiraoka, L.R., Harrington, J.J., Gerhard, D.S., Lieber, M.R. and Hsieh, C.L. (1995) Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human. Genomics 25:220-5.
2. Warbrick, E., Coates, P.J. and Hall, P.A. (1998) Fen1 expression: a novel marker for cell proliferation. J Pathol 186:319-24.
3. Kim, K., Biade, S. and Matsumoto,Y. (1998) Involvement of flap endonuclease 1 in base excision DNA repair. J Biol Chem 273:8842-8.
4. Rumbaugh, J.A., Henricksen, L.A., DeMott, M.S. and Bambara, R.A. (1999) Cleavage of substrates with mismatched nucleotides by flap endonuclease-1. Implications for mammalian Okazaki fragment processing. J Biol Chem 274:14602-8.
5. Kolodner, R.D. and Marsischky, G.T. (1999) Eukaryotic DNA mismatch repair. Current Opinion Genet Dev 9:89-96.