Technical Data
Media
Fischer’s Medium (Leukemic Cells of Mice) w/L-Glutamine (Powder)
F0100
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•2H2O91.000
Magnesium Chloride•6H2O100.000
Potassium Chloride400.000
Sodium Chloride8000.000
Sodium Phosphate Dibasic60.000
Sodium Phosphate Monobasic60.000
Amino Acids:
L-Arginine•HCl15.000
L-Asparagine10.000
L-Cystine•2HCl26.100
L-Glutamine204.000
L-Histidine•HCl•H2O81.000
L-Isoleucine75.000
L-Leucine30.000
L-Lysine•HCl50.000
L-Methionine100.000
L-Phenylalanine60.000
L-Serine15.000
L-Threonine40.000
L-Tryptophan10.000
L-Tyrosine•2Na•2H2O87.160
L-Valine70.000
Vitamins:
D-Biotin0.010
Choline Chloride1.500
Folic Acid10.000
myo-Inositol1.500
Niacinamide0.500
D-Pantothenic Acid•Ca0.500
Pyridoxal•HCl0.500
Riboflavin0.500
Thiamine•HCl1.000
Other:
D-Glucose1000.000
Phenol Red, Sodium5.300
Total:10.606g/L
Fischer’s Medium was originally formulated to support serial propagation of cells from leukemic mice. In initial studies, cells in culture were examined for resistance to chemotherapeutic agents. While most of such studies were carried out using whole animals, Fischer’s Medium made it possilbe to conduct parallel studies in vitro. Fischer’s Medium supports clonal reproduction of cells, particularly lymphoblasts from primary explants or from cells in culture.


Directions per Liter: Dissolve 10.6g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add 1.125g sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to pale orange, homogeneous, free flowing powder

Solubility (10.6g/L): Orange, clear, complete

pH: 6.6 ± 0.4

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Fischer, G.A. and Sartorelli, A.C. Methods In Med. Res. 10:47 (1964)