Technical Data
F3965-07
FEZ2, IN (Fasciculation and elongation protein zeta 2, HUM3CL, MGC117372, Pre-T/NK cell-associated protein 3Cl, Zygin-2, Zygin II)
Description:
Fasciculation and elongation protein zeta-2 (FEZ2) is a homolog to the mammalian FEZ1, itself an ortholog of the C. elegans UNC-76 (1,2). In contrast to FEZ1, FEZ2 mRNA is widely expressed in mouse tissues. FEZ2 interacts with protein kinase C (PKC)- substrate and induces neurite extension of PC12 cells when co-expressed with a constitutively active form of PKC- , suggesting FEZ2 may play an important role in the morphological changes of various cells by associating with PKC- in a tissue non-specific manner (1). FEZ2 can interact with FEZ1 through its c- terminal regions and especially its coiled-coil region (5). At least two isoforms of FEZ2 are known to exist.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5–1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to 15aa from near the center of human FEZ2 (Genbank accession No. NP_001036013).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human FEZ2. Species crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fujita T, Ikuta J, Okajima T, et al. Identification of a tissue- non-specific homologue of axonal fasciculation and elongation protein zeta-1. Biochem. Biophys. Res. Commun. 2004; 313:738-44. 2. Kuroda S, Nakagawa N, Tokunaga C, et al. Mammalian homologue of the Caenorhabditis elegans UNC-76 protein involved in axonal outgrowth is a protein kinase C -interacting protein. J. Cell Biol. 1999; 144:403-11. 3. Assmann EM, Alborghetti MR, Camargo MER, et al. FEZ1 dimerization and interaction with transcription regulatory proteins involves its coiled-coil region. J. Biol. Chem. 2006; 281:9869-81.