Technical Data
F4015-04D
Ferritin
Description:
Ferritin is important in iron homeostasis. It is the main iron-storage protein, composed of two partially homologous subunits, heavy and light chains. Ferritin molecules in cells containing high levels of iron tend to be rich in light chains, and may have a long-term storage function, whereas heavy-rich ferritins are more active in iron metabolism. Mutations in the ferritin gene cause the hereditary hyperferritinemia-cataract syndrome and neuroferritinopathy, associated with inflammation. Elevated levels of ferritin are reported as characteristic of adult-onset Still's disease and hemophagocytic syndrome, also associated with inflammation. Microfilaments and increased levels of exogenous ferritin are excreted directly into the bile by way of a second microfilament-independent, chloroquine-insensitive pathway, supporting a possible physiological mechanism for the release of ferritin from the liver.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Matched Pairs:
Capture: F4015-04D
Detection: F4015-04C, F4015-04E, F4015-04B, F4015-04D. F4015-04I

Affinity Constant:
4x10e10 L/mole

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k9L245Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanMouse
Concentration:
~5mg/ml
Immunogen:
Ferritin isolated from human liver.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM phosphate, pH 7.4, 150mM sodium chloride, 0.1% sodium azide.
Specificity:
Recognizes human Ferritin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Englebienne, P., et al., (2000), "Rapid Homogeneous Immunoassay for Human Ferritin in the Cobas Mira Using Colloidal Gold as the Reporter Reagent", Clinical Chemistry, 46(12): 2000-2003.