Technical Data
F4015-13B
FTL (Ferritin, Light Polypeptide, MGC71996, L apoferritin, Ferritin light polypeptide-like 3)
Description:
FTL is the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Peptide ELISA: 1:4000
Sandwich ELISA: 1-2ug/ml.
Western Blot: 0.1-0.3ug/ml. Band observed at ~20kD in human brain (Cerebellum), liver and placenta lysates.
Immunohistochemistry (Paraffin): 3-6ug/ml shows cytoplasm staining of pyramydal neurons in human brain cortex.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-GEYLFERLTLKHD from the C-terminus of human FTL (Accession #NP_000137.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human FTL. Species Sequence Homology: mouse, rat, canine and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sammarco MC, Ditch S, Banerjee A, Grabczyk E.Ferritin L and H subunits are differentially regulated on a post-transcriptional level. J. Biol. Chem. 2008 Feb 283 (8): 4578-87. PMID: 18160403