		Technical Data
F4100-12X	a-Fetoprotein (AFP, Alpha1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP) (BSA & Azide Free)

Description:
AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:10-1:20 for 30 minutes at RT. No special pretreatment is required for staining of formalin fixed, paraffin embedded tissues.
Optimal dilutions to be determined by the researcher.

Positive Control:
Fetal liver or hepatocellular carcinoma.

Source:
Ascites

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	0.N.13	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Affinity purified alpha fetoprotein from serum of hepatoma patient. Cellular Localization: Cytoplasmic.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4. No preservatives or stabilizing proteins added. Also available with BSA and azide. See F4100-12.

Specificity:

Recognizes human alpha fetoprotein (AFP) at 70kD. Does not react with other onco-fetal antigens or serum albumin. Species Crossreactivity: canine and porcine. Does not react with bovine, feline, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Yazova, A.K., et al., Immunology Letters, Sep, 25(4):325-30 (1990). 2. Cocquyt, V., et al., American Journal of Clinical Oncology, 19(5):465-8 (1996). 3. Morinaga, S., et al., Archives of Pathology and Laboratory Medicine, Jul, 120(7):687-90 (1996). 4. Martin de las Mulas, J., et al., Research in Veterinary Science, 59(2):124-7 (1995). 5. Shinagawa, T., et al., Virchows Archiv, 426(4):419-23 (1995). 6. Aizawa, K., et al., Int Journal of Cancer, 58(3):430-5 (1994). 7. Cummings, O.W., et al., Human Pathology, 25:54-9 (1994). 8. Fucich, L.F., et al., Archives of Pathology and Laboratory Medicine, Sep, 118(9):927-30 (1994). 9. Kawamori, T., et al., Clinical Investigator, May, 72(5):377-80 (1994).