Technical Data
Fetuin (alpha-2 Heremans-Schmid Glycoprotein, FHSG, Fetuin A)
Molecular Biology Storage: -20CShipping: Blue Ice
Fetuin is a liver-produced negative acute phase protein composed of two subunits, the A and B chains Fetuin homologs have been identified in several species including rat, sheep, pig, rabbit, guinea pig, cattle, mouse and human. Multiple physiological roles for these homologs have been suggested, including ability to bind to hydroxyapatite crystals and to specifically inhibit the tyrosine kinase (TK activity of the insulin receptor (IR). Fetuin-A (alpha2-Heremans-Schmid glycoprotein; AHSG) is an important circulating inhibitor of calcification in vivo, and is downregulated during the acute-phase response. Sera from patients on longterm dialysis with low AHSG concentrations showed impaired ex-vivo capacity to inhibit CaxPO4 precipitation. Fetuin may influence the resolution of inflammation by modulating the phagocytosis of apoptotic cells by macrophages. ASHG blocks TGF-beta-dependent signaling in osteoblastic cells, and
mice lacking ASHG display growth plate defects, increased bone formation with age, and enhanced cytokine-dependent osteogenesis.

Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Reconstitution: Reconstitute in 0.2ml sterile ddH2O. Allow the lyophilized pellet to dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the protein. Clarify by centrifugation.

Storage and Stability:
Lyophilized powder may be stored at 4C for up 2 weeks only. Reconstitute to nominal volume by adding sterile ddH2O. Aliquot and store reconstituted protein at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Human plasma
Purity: 95% (14% SDS-PAGE separation of human fetuin, 5ug/lane)
Concentration: ~0.5mg/ml
Form: Supplied as a lyophilized powder in 0.02M Tris-HCl, pH8.0, 0.2M sodium chloride. No preservatives added.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Denecke B, Graber S, Schafer C, Heiss A, Woltje M, Jahnen-Dechent W. Tissue distribution and activity testing suggest a similar but not identical function of fetuin-B and fetuin-A. Biochem J. 2003 Nov 15; 376(Pt 1): 135-45. 2. Schafer C, Heiss A, Schwarz A, Westenfeld R, Ketteler M, Floege J, Muller-Esterl W, Schinke T, Jahnen-Dechent W. The serum protein alpha 2-Heremans-Schmid glycoprotein/fetuin-A is a systemically acting inhibitor of ectopic calcification. J Clin Invest. 2003 Aug; 112(3): 357-66. 3. Jersmann HP, Dransfield I, Hart SP. Fetuin/alpha2-HS glycoprotein enhances phagocytosis of apoptotic cells and macropinocytosis by human macrophages. Clin Sci (Lond). 2003 Sep; 105(3): 273-8. 4. Ketteler M, Bongartz P, Westenfeld R, Wildberger JE, Mahnken AH, Bohm R, Metzger T, Wanner C, Jahnen-Dechent W, Floege J. Association of low fetuin-A (AHSG) concentrations in serum with cardiovascular mortality in patients on dialysis: a cross-sectional study. Lancet. 2003 Mar 8; 361(9360): 827-33. 5. Arnaud P, Kalabay L. Alpha2-HS glycoprotein: a protein in search of a function. Diabetes Metab Res Rev. 2002 Jul-Aug; 18(4): 311-4. Review.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.