Technical Data
F4149-74
Flh, CT (Floating Head, Homeobox Protein)
Description:
Floating head (Flh) is a zebrafish homeobox gene critical to notochord development, and is one of the earliest genes to be expressed in notochord precursors. Studies have shown that Flh is critical to epiphysial neurogenesis, glomerular differentiation, axial vascular development and other key elements of zebrafish growth. Zebrafish Flh mutants develop without a notochord, but still develop with mesodermal derivatives such as the prechordal plate. In Flh mutants, Flh RNA is expressed at lower levels, suggesting a mechanism of positive self-regulation. Flh is a homologue of the homeobox gene Xnot in amphibians.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:20,000
Optimal dilutions to be determined by the researcher.

Control Peptide: F4149-74P

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceZebrafishRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the C-terminal region of zebrafish Flh.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
Specificity:
Recognizes zebrafish Flh.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dev. Dyn. 209(2):156-165 2. Majumdar, A and Drummond, IA (2000) Neuron 18(1):43-57. 3. Cau, E and Wilson, SW (2003) Development 130(11):2455-2466. 4. Sumoy, L et al (2000) Mech. Dev. 63(1):15-27. 5. Talbot, WS et al (1995) Nature 378:150-157.