		Technical Data
F4165-01	FIAF (Fasting-induced Adipose Factor, Angiopoietin-like Protein 4, ANGPTL 4, PPAR gamma Angiopoitein-related Protein, PGAR, Hepatic Fibrinogen Angiopoietin-related Protein, HFARP)

Description:
The fasting-induced adipose factor (FIAF, ANGPTL4, PGAR, HFARP) was previously identified as a novel adipocytokine that was up-regulated by fasting, by peroxisome proliferators- activated receptor agonists, and by hypoxia. FIAF and other adipocytokines including resistin, adiponectin, acylationstimulating protein, plasminogen activator inhibitor-1, rennin angiotensin system, metallothioneins and tumor growth factor-b have been implicated in a variety of different processes, ranging from blood pressure control to lipid metabolism and insulin senziting. It has been tempting to attribute many of the clinical abnormalities associated with obesity from insulin resistance to altered secretion of particular adipocytokines.

Amino acid sequence:
MRGSHHHHHH GMASHMGPVQ SKSPRFASWD EMNVLAHGLL QLGQGLREHA ERTRSQLSAL ERRLSACGSA CQGTEGSTDL PLAPESRVDP EVLHSLQTQL KAQNSRIQQL FHKVAQQQRH LEKQHLRIQH LQSQFGLLDH KHLDHEVAKP ARRKRLPEMA QPVDPAHNVS RLHRLPRDCQ ELFQVGERQS GLFEIQPQGS PPFLVNCKMT SDGGWTVIQR

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: Indirect
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Recombinant peptide corresponding to 220aa and the N-terminal His-tag (16aa) of human ANGPTL4. MW of Antigen: 25kD. Sequence homology: Human ANGPTL4 precurser (aa22-229) without signal sequence: 100%.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Reconstitute with 100ul sterile ddH2O.

Specificity:

Recognizes human Angiopoietin-Like Protein.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kersten S.; Mandard S.; et al.: Characterization of the Fasting-induced Adipose Factor FIAF, a Novel Peroxisome Proliferator-activated Receptor Target Gene. J. of Biological Chemistry (2000) Septemper 15; 275 (37): 28488-28493. 2. Mandard S.; Zandbergen F.; Soon N.S.; et al: The direct peroxisome proliferator-activated receptor target fasting-induced adipose factor (FIAF/PGAR/ANGPTL4) is present in blood plasma as a truncated protein that is increased by fenofibrate treatment. J. of Biological Chemistry (2004) Aug 13; 279 (33): 34411-34420