Technical Data
F4193-02
Fibrillarin (FBL, 34kD Nucleolar Scleroderma Antigen, rRNA 2'-O-methyltransferase fibrillarin, FLRN, FIB1, Nop1p, RNU3IP1)
Description:
Nop1p/Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologues to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of two extended beta-sheets flanked by four alpha-helices. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34,000kD protein, which has been subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.
Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:500-1:2500 for yeast fibrillarin; 1:250 for mammalian fibrillarin.
Western Blot: 1:1000 (cell lysates)-1:5000 (nuclear fractions) for yeast fibrillarin; 1:250 for mammalian fibrillarin. ECL detection was used.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG8A152Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceMouse
Concentration:
~1mg/ml
Immunogen:
Yeast fibrillarin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity:
Recognizes yeast fibrillarin. Species crossreactivity: human, rat, plant, Drosophila, C. elegans and S. pombe.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tam R, et al. The 4q subtelomere harboring the FSHD locus is specifically anchored with peripheral heterochromatin unlike most human telomeres. J. Cell Biol. Oct 2004; 167(2): 269-279. 2. Partridge JJ, et al. DNA damage modulates nucleolar interaction of the Werner protein with the AAA ATPase p97/VCP. Mol. Biol. Cell. Oct 2003; 14(10): 4221-4229. 3. Pollard KM, et al. Proteolytic cleavage of a self-antigen following xenobiotic-induced cell death produces a fragment with novel immunogenic properties. J. Immunol. Aug 2000; 165(4): 2263-2270.