Technical Data
Fibrillarin (rRNA 2'-O-methyltransferase Fibrillarin, D2870, LOT3, Nucleolar Protein 1, NOP1, Nop1p, U3 Small Nucleolar RNA-associated Protein NOP1, U3 snoRNA-associated Protein NOP1, YDL014W)
Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal ~80 amino acids contain multiple copies based on the peptide RGG and the remaining ~240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologues to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended b-sheets flanked by 4 alpha helixes. Patients with the autoimmune disease scleroderma often have circulating auto-antibodies to a 34,000D protein which was subsequently found to be fibrillarin.

Suitable for use in Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blots of yeast protein samples: 1:5000 (cell lysates) to 1:25,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) Western detection methods: 1:5000 to 1:2500.
Immunofluoscence on yeast cells: 1:1000-1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Mab8A226Affinity Purified
100ug-20CBlue IceMouse
Yesat (S. cerevisiae) fibrillarin
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Recognizes yeast (S. cerevisiae) Nop1p. Does not crossreact with fibrillarin in other species, but is an excellent marker of the nucleous or yeast cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pintard L, et al. Spb1p is a yeast nucleolar protein associated with Nop1p and Nop58p that is able to bind S-adenosyl-L-methionine in vitro. Feb 2000; 20(4): 1370-1381.