		Technical Data
F4193-02B	Fibrillarin (FBL, 34kD Nucleolar Scleroderma Antigen, rRNA 2'-O-methyltransferase fibrillarin, FLRN, FIB1, Nop1p, RNU3IP1)

Description:
Nop1p/Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232°C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologues to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended b-sheets flanked by 4 a-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34,000 Da protein which was subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

Applications:
Suitable for use in Wester Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blots of yeast protein samples: 1:1000 (cell lysates) to 1:5000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) Western detection methods: 1:500 to 1:2500. To detect mammalian fibrillarin on Western Blots by ECL: 1:250.
Immunofluoscence on yeast cells: 1:500-1:2500. For IF of mammalian cells: 1:250.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab		8A227	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

~1mg/ml

Immunogen:

Yeast fibrillarin

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

Specificity:

Reacts with fibrillarin in human, rat, plant, Drosophia, C. elegans and S. pombe.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Levitskii SA, et al. Identification of signal sequences determining the specific nucleolar localization of fibrillarin in HeLa cells. Mol. Biol. (Mosk). May-Jun 2004; 38(3): 483-492. 2. Newton K, et al. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol. Cell Biol. Dec 2003; 23(23): 8519-8527.