Technical Data
F4193-02C
Fibrillarin (FBL, 34kD Nucleolar Scleroderma Antigen, rRNA 2'-O-methyltransferase fibrillarin, FLRN, FIB1, Nop1p, RNU3IP1)
Description:
Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

Applications:
Suitable for use in Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blotting: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18C147Affinity Purified
SizeStorageShippingSourceHost
50ug4C/-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Partial sequence of recombinant full-length protein to human Fibrillarin
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Specificity:
Recognizes human Fibrillarin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.