Technical Data
F4194-05B
Fibrillin (Biotin)
Description:
The fibrillins are extracellular matrix glycoproteins which occur as a major component of a subset of connective tissue microfibrils. These microfibrils have a beaded appearance and a cross-sectional diameter of 10-12nm. In elastic tissues, these structures are thought to provide the scaffold onto which elastin is assembled to form elastic fibers, although their function in non-elastic tissues is unclear.1 In immunohistochemical staining of paraformaldehyde-fixed human skin, mAb labels the elastic framework and more specifically the oxytalantic network close to the epidermis. The antibody also exhibits discrete staining of normal fibroblasts in culture and of extracellular (elastin) fibrils in a variety of tissues.2,3 There is an aberrant expression of fibrillin in Marfan syndrome.4

Applications:
Immunohistochemistry 2
Immunofluorescence 2
Western Blot 2
Electron microscopic immunolocalization 2

Recommended Dilution:
Immunohistochemistry: 1:50-1:100 (30-60 min at RT)
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k5K135Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceBovineMouse
Concentration:
~0.2mg/ml
Immunogen:
Microfibrils from zonular apparatus of bovine eye
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
Specificity:
Recognizes bovine fibrillin (Mr 350kD). Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.