Technical Data
F4195-01
Fibrillin 1
Description:
Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule. Fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils. There is an aberrant expression of fibrillin-1 in Marfan syndrome.

Applications:
Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and ELISA. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q627Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human Fibrillin 1
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes human Fibrillin 1. Epitope mapping studies identify the binding site of this antibody to the amino-terminal end of the molecule, between aa45-450. Species Crossreactivity: chicken and bovine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Reinhardt, D., et al. (1996) J. Mol. Biol. 258: 104–116. 2. Keene, D., et al. (1997) J. Histochem. Cytochem. 45: 1069–1082.