Technical Data
F4195-05A
Fibrillin 1, exons 36-44
Description:
Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule. Fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils. There is an aberrant expression of fibrillin-1 in Marfan syndrome.

Applications:
Suitable for use in Immunohistochemistry, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:2000
Western Blotting: 1:200-1:500
Immunohistochemistry: 1:50-1:100
Immunoprecipitation: 1:100-1:200
Optimal dilutions to be determined by the researcher.

Note:
In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Protein fragment containing exons 36-44 of human fibrillin-1.
Purity:
Serum
Form
Supplied as a lyophilized powder. No preservative added.
Specificity:
Reacts strongly with human and bovine fibrillin-1. Crossreactivity with fibrillin-2 has not been determined.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Pereira et al. Human Mol. Gen. 2:961 (1993).