Technical Data
F4199-90B
Fibrinogen (Coagulation Factor I) (FITC)
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Technical Data |
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F4199-90B |
Fibrinogen (Coagulation Factor I) (FITC) |
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Description: Fibrinogen is synthesized in the liver and platelets and plays a major role in the coagulation process. It consists of 6 polypeptide chains, two each of A alpha-, B beta- and gamma-chains and is a dimeric molecule, each subunit consisting of one of each chain type. Thrombin cleaves N-terminal peptides from each of the A alpha-and B beta-chains to form fibrin monomer. The newly exposed N-terminal sites spontaneously link to C-terminal sites of another fibrin molecule to form a fibrin chain. These are then crosslinked by activated Factor XIII which stabilizes the fibrin clot. Normal plasma levels of fibrinogen are around 2.6g/L. Labeling Ratio: Fluorescein:antibody molar ratio: 2.8-3.3 Absorbance ratio (A493/A276): 0.78±0.05 Applications: Suitable for use in Immunofluorescence (direct or indirect) and Fluorescence microscopy. Other applications not tested. Recommended Dilution: 1. Incident Light Excitation Direct Immunofluorescence: 1:10-1:100 Indirect Immunofluorescence: 1:20-1:400 2. Transmitted Light Excitation Direct Immunofluorescence: 1:5-1:40 Indirect Immunofluorescence: 1:10-1:80 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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