Technical Data
F4199-90B
Fibrinogen (Coagulation Factor I) (FITC)
Description:
Fibrinogen is synthesized in the liver and platelets and plays a major role in the coagulation process. It consists of 6 polypeptide chains, two each of A alpha-, B beta- and gamma-chains and is a dimeric molecule, each subunit consisting of one of each chain type. Thrombin cleaves N-terminal peptides from each of the A alpha-and B beta-chains to form fibrin monomer. The newly exposed N-terminal sites spontaneously link to C-terminal sites of another fibrin molecule to form a fibrin chain. These are then crosslinked by activated Factor XIII which stabilizes the fibrin clot. Normal plasma levels of fibrinogen are around 2.6g/L.

Labeling Ratio:
Fluorescein:antibody molar ratio: 2.8-3.3
Absorbance ratio (A493/A276): 0.780.05

Applications:
Suitable for use in Immunofluorescence (direct or indirect) and Fluorescence microscopy. Other applications not tested.

Recommended Dilution:
1. Incident Light Excitation
Direct Immunofluorescence: 1:10-1:100
Indirect Immunofluorescence: 1:20-1:400

2. Transmitted Light Excitation
Direct Immunofluorescence: 1:5-1:40
Indirect Immunofluorescence: 1:10-1:80

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
2ml4C (-20C Glycerol)Blue IceHumanSheep
Concentration:
Not determined
Immunogen:
Purified fibrinogen derived from pooled human plasma.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 20mM Tris, 70mM sodium chloride, pH8.0, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Prior to conjugation, the antibody was specific for fibrinogen when tested against human plasma by Coomassie Blue R stained double immunodiffusion and immunoelectrophoresis. Undesired immune reactivity has been removed by solid phase immunoadsorption.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.