Technical Data
F4199-91
Fibrinogen (Coagulation Factor I)
Description:
Fibrinogen is an abundant plasma protein (5-10uM) produced in the liver. The intact protein has a molecular weight of 340kD and is composed of 3 pairs of disulfide-bound polypeptide chains named Aalpha, Bbeta and gamma. Fibrinogen is a triglobular protein consisting of a central E domain and terminal D domains.

Applications:
Suitable for use in ELISA, Western Blot and RIA. Other applications have not been tested.

Recommended Dilutions:
ELISA: Binds human fibrinogen in solid-phase format.
Western Blot (Non-reduced): Binds A-alpha chain of fibrinogen.
RIA: Binds 125I-labeled fibrinogen.
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS-1 myeloma cells with spleen cells from Balb/c mice.

Source:
Culture

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG11.B.697Affinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Human fibrinogen
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from 10mM sodium phosphate, 0.15M sodium chloride, pH 7.4, 0.01% sodium azide. Reconstitute with 500ul sterile ddH2O.
Specificity:
Recognizes human fibrinogen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.