Technical Data
F4199-91B
Fibrinogen (Coagulation Factor I)
Description:
Fibrinogen, the precursor of fibrin, is the coagulable protein in the blood plasma of vertebrates. Fibrinogen consists as a dimer of 3 pairs of non-identical chains, alpha (Mr= 66,800), beta (Mr= 52,000) and gamma (Mr= 46,500) cross-linked by disulfide bonds in their N-terminal segments. The molecule has 2 terminal D domains and one central E domain, all three domains are separated when fibrinogen is degradated by plasmin. MW is 340kD (1). Concentration in human plasma is 2-3mg/ml (2).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000
Western Blot: Reacts with fibrinogen in non-reduced form only.
Optimal dilutions to be determined by the researcher.

Culture Medium:
RMPI 1640 with 2-10% fetal calf serum.

Hybridoma:
X63-Ag8.653. Female CF1 x Balb/c mice

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k6F99Affinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Fibrinogen isolated from human plasma.
Purity:
Purified by Protein A/G affinity chromatography from culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide. No stabilizing proteins added.
Specificity:
Recognizes human Fibrinogen. Reacts strongly with fibrinogen. Strong reaction is seen in ELISA with factor VIII HP directly coated onto the microtiterwell, also when tested in sandwich ELISA in combination with a polyclonal antibody against fibrinogen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Doolittle RF Structure and function of fibrinogen. Horiz Biochem Biophys 3:164-191.(1977). 2. Scott T & Eagleson M Concise Encyclopedia Biochemistry: Walter de Gruyter, New York. (1988).