Technical Data
F4199-91E
Fibrinogen (Coagulation Factor I)
Description:
Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking.

Applications:
Suitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion (Ouchterlony). Other applications not tested.

Recommended Dilution:
ELISA: 1:500-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
10mg-20CBlue IceCanineGoat
Concentration:
Not determined
Immunogen:
Fibrinogen isolated from fresh plasma after removing prothrombin
Purity:
Purified
Form
Supplied as a lyophilized powder from PBS, pH 7.2, No preservative added. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity:
Recognizes canine fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), against normal canine plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. May also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. Does not crossreact with any other component of canine plasma.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.