Technical Data
F4199-92
Fibrinogen (Coagulation Factor I)
Description:
Fibrinogen is synthesized in the liver and platelets and plays a major role in the coagulation process. It consists of 6 polypeptide chains, two each of A alpha-, B beta- and gamma-chains and is a dimeric molecule, each subunit consisting of one of each chain type. Thrombin cleaves N-terminal peptides from each of the A alpha-and B beta-chains to form fibrin monomer. The newly exposed N-terminal sites spontaneously link to C-terminal sites of another fibrin molecule to form a fibrin chain. These are then crosslinked by activated Factor XIII which stabilizes the fibrin clot. Normal plasma levels of fibrinogen are around 2.6g/L.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Recommended Pairs:
Capture: F4199-92, F4199-92E
Detection: F4199-92D, F4199-92

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG15E598Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Fibrin degradation products
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human Fibrinogen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.