Technical Data
F4199-92K
Fibrinogen (Coagulation Factor I) (HRP)
Description:
Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the
precursor of fibrin, which is the key protein constituting the network of the blood clot.
Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize
to fibrin which is stabilized by cross-linking.

Applications:
Suitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion. Other applications not tested.

Recommended Dilution:
ELISA: 1:500-1:4000
Optimal dilutions to be determined by the researcher.

Peroxidase/IgG protein molar ratio (E/P): ~1.7

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceRatGoat
Concentration:
Not determined
Immunogen:
Fibrinogen isolated from fresh rat plasma after removing prothrombin
Purity:
Purified by DEAE chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labeled with Horseradish Peroxidase (HRP). Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity:
Recognizes rat Fibrinogen. In immunoelectrophoresis and radial immunodiffusion against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein components or serum. May also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. Does not crossreact with any other component of rat plasma.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.