Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the
precursor of fibrin, which is the key protein constituting the network of the blood clot.
Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers
polymerize to fibrin which is stabilized by cross-linking.
Suitable for use in IEP and RID. Other applications have not been tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not determined |
|Fibrinogen is isolated from fresh plasma after removing prothrombin.|
|Supplied as a lyophilized powder. No prevervatives added. Reconstitute with 1ml streil ddH2O. Dilute further with PBS. |
|Recognizes canine Fibrogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal dog plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. The antiserum does not cross react with any other component of dog plasma. Inter- species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|