Technical Data
Fibrinogen, Citrullinated (FGA, FGB, FGG, Fibrinogen A alpha Polypeptide Chain, Fibrinogen alpha Chain, Fibrinogen beta Chain, Fibrinogen gamma Chain, MGC104327, MGC119422, MGC119423, MGC119425, MGC120405)
Fibrinogen is a protein produced by the liver which helps stop bleeding by helping blood clots to form. Fibrinogen gets deiminated (conversion from arginin to citrullin) by Peptidyl Arginine Deminase (PAD) in inflamed joints in patients that develop rheumatoid arthritis. Citrulline, while being an amino acid, is not built into proteins during protein synthesis, as it is not coded for by DNA, yet several proteins are known to contain citrulline. Proteins that normally contain citrulline residues include myelin basic protein (MBP), filaggrin, and several histone proteins, while other proteins, like fibrin and vimentin can get deiminated during cell death and tissue inflammation. Patients with rheumatoid arthritis often (at least 80% of them) develop an immune response against proteins containing citrulline. Although the origin of this immune response is not known, detection of antibodies reactive with citrulline containing proteins or peptides is now becoming an important help in the diagnosis of rheumatoid arthritis.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS or Tris-HCl. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG120B2Affinity Purified
100ug-20CBlue IceMouseMouse
~0.5mg/ml (after reconstitution)
Deiminated mouse fibrinogen peptide.
Purified by Protein A affinity chromatography.
Supplied as a lyophilized powder from 10mM ammonium bicarbonate, 1% BSA. Reconstitute with 200ul sterile PBS or Tris-HCl, pH 7-8.
Recognizes deiminated mouse fibrinogen. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.