Technical Data
F4210-08A
Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)
25ug
Growth Factors, Cytokines Storage: -20°CShipping: Blue Ice
Growth factor additive in cell culture media for mesoderm and neuroectoderm-derived cells. Half maximum stimulation of cell proliferation with bovine aortic endothelial cells (BAE cells) is reached using < 3ng/ml.

Biological Activity:
3 ng/ml for half maximum stimulation of cell proliferation with bovine aortic endothelial cells (BAE cells).

Applications:
Characterized growth factor additive in cell culture media for mesoderm and neuroectoderm-derived cells.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
20kD
 Source: Human recombinant, produced in E. coli
Purity: 95%; Purified by Heparin affinity chromatography.
Concentration: ~0.2mg/ml
Form: Lyophilized in 100ul phosphate buffered saline (pH 6), supplemented with 5mM glutathione. Reconstitute with 100ul distilled water.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Thomas KA et al: Purification and characterization of acidic fibroblast growth factor from bovine brain. Proc Natl Acad Sci USA 81, 357- 361 (1984)
Böhlen P et al: Acidic fibroblast growth factor (FGF) from bovine brain; amino-terminal sequence and comparison with basic FGF. EMBO J 4, 1951-1965 (1985)
Gimenez-Gallego G et al: Brain-derived acidic fibroblast growth factor: Complete amino acid sequence and homologies. Science 230, 1385-1388 (1985)
Jaye M et al: Human endothelial cell growth factor: Cloning, nucleotide sequence and chromosome localization. Science 233, 541-545 (1986)
Mergia A et al: The genes for basic and acidic fibroblast growth factors are on different human chromosomes. Biochem Biophys Res Comm 138, 644-651 (1986)
Linemeyer D et al: Expression in E. coli of a chemically synthesized gene for biologically active, bovine acidic fibroblast growth factor. Biotechnology 5, 960-964 (1987)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.