Technical Data
Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)
Molecular Biology Storage: -20CShipping: RT
Fibroblast Growth Factor-acidic (FGF-a or FGF-1) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. Human FGF-acidic is a 15.8kD protein containing 140 amino acid residues

Biological Activity:
Human FGF-a is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors if 10ng/ml, corresponding to a specific activity of 1x10e5 units/mg.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile buffer and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 95% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug
Form: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute in 5mM sodium phosphate, pH 7.0-7.2, to a concentration of < 0.5mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.