Technical Data
Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)
Molecular Biology Storage: -20CShipping: RT
Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Recombinant Human Fibroblast Growth Factor-acidic (FGF-1) is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15,967 Daltons.

Amino Acid Sequence:

Biological Activity:
The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells is <1ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Recombinant, Human expressed in E. coli
Purity: 97% as determined by: RP-HPLC and SDS-PAGE. Chromatographically purified.
Form: Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with sterile ddH2O to a concentration of 0.1-0.25mg/ml. Allow sample to sit for 5 minutes at 4C, spin to remove precipitant.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Phosphorylation-regulated Nucleocytoplasmic Trafficking of Internalized FGF-1. Wiedlocha A, Nilsen T, Sorensen V, Mol Biol Cell 2004 Dec 1; 2. Incorporation of polymer microspheres within fibrin scaffolds for the controlled delivery of FGF-1. Royce SM, Askari M, J Biomater Sci Polym Ed 2004;15(10):1327-36 3. Role of AKT/PKB signaling in fibroblast growth factor-1 (FGF-1)-induced angiogenesis in the chicken chorioallantoic membrane (CAM). Forough R, Weylie B, Ambrus S, J Cell Biochem 2005 Jan 1;94(1):109-16 4. Testicular FGF-1 protein is involved in Sertoli cell-spermatid interaction in roe deer. Schon J, Blottner S, Gen Comp Endocrinol 2004 Nov;139(2):168-72 5. Design of fully active FGF-1 variants with increased stability. Zakrzewska M, Krowarsch D, Otlewski J, Protein Eng Des Sel 2004 Aug;17(8):603-11 6. Human FGF-1 gene transfer promotes the formation of collateral vessels and arterioles in ischemic muscles of hypercholesterolemic hamsters. Caron A, Michelet S, Sordello S, Schwartz B, J Gene Med 2004 Sep;6(9):1033-45

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.