Technical Data
F4210-10D
Acidic Fibroblast Growth Factor, Recombinant, Human (aFGF)
100ug
500ug
500ug
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Technical Data |
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F4210-10D |
Acidic Fibroblast Growth Factor, Recombinant, Human (aFGF) |
100ug 500ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Acidic fibroblast growth factor(aFGF) belongs to the fibroblast growth factor family. It is a potent mitogenic agent. aFGF has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade. Recombinant human aFGF was overexpressed in E. coli and purified by conventional chromatography. Molecular Weight: 15.9kD (141aa) Amino Acid Sequence: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 15.9kD |
Source: Recombinant, Human, produced in E. coli Purity: 95% by SDS PAGE Concentration: ~1mg/ml Form: Supplied as a liquid in 20mM Tris, pH 7.0, 100mM sodium chloride. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Jaye M. et al., (1986) Science. 233: 541-544. 2. Mergia A. et al., (1989) Biochem. Biophys. Res. Commun. 164:1121-1129. 3. Stauber DJ. et al., (2000) Proc. Natl. Acad. Sci. U.S.A. 97: 49-54.
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