Technical Data
F4210-10J
Fibroblast Growth Factor, Acidic, Recombinant, Human (Acidic Fibroblast Growth Factor, aFGF, FGF-alpha, FGFA, Beta-endothelial Cell Growth Factor, ECGF-beta, ECGFB, ECGF, ECGFA, FGF1, GLIO703, Heparin-binding Growth Factor 1, HBGF1, HBGF-1)
50ug
Molecular Biology Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to 155aa of human Fibroblast Growth Factor-acidic expressed in E.coli is a single, non-glycosylated,polypeptide chain.

Molecular Weight:
~17.5kD

Biological Activity:
The ED50, calculated by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is less then 10ng/ml, corresponding to a specific activity of 10Units/mg.

Dimers and Aggregates:
1% (SDS-PAGE)

Protein Content:
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a standard solution of FGF-acidic as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~17.5kD
Purity: ~95% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Immunolocalization of fibroblast growth factor-1 (FGF-1), its receptor (FGFR-1), and fibroblast-specific protein-1 (FSP-1) in inflammatory renal disease. Kidney Int 2005 Dec;68(6):2621-8. 2. Molecular Mechanism of Inhibition of Nonclassical FGF-1 Export(,). Biochemistry 2005 Nov 29;44(47):15472-15479. 3. An experimental and molecular-modeling study of the binding of linked sulfated tetracyclitols to FGF-1 and FGF-2. Chembiochem 2005 Oct;6(10):1882-90. 4. FGF-1 and S100A13 possibly contribute to angiogenesis in endometriosis. J Reprod Immunol 2005 Oct;67(1-2):87-101. 5. Effects of exogenous FGF-1 treatment on regeneration of the lens and the neural retina in the newt, Notophthalmus viridescens. J Exp Zoolog A Comp Exp Biol 2005 Oct 1;303(10):837-44. 6. Fibroblast Growth Factor (FGF)-1, FGF2, FGF7 and FGF Receptors are Uniformly Expressed in Trophoblast Giant Cells During Restricted Trophoblast Invasion in Cows. Placenta 2005 Aug 26;

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.