Technical Data
F4210-14P
Fibroblast Growth Factor, Basic (FGFb, BFGF, Fibroblast Growth Factor 2, FGF2, Heparin-binding Growth Factor 2, HBGF-2)
Description:
Fibroblast growth factors (FGFs), a heparin binding growth factor, exhibit widespread mitogenic and neurotrophic activities in a variety of different cells including mesenchymal, neuroectodermal and endothelial cells. aFGF (FGF-1) and bFGF (FGF-2) are present in relatively high levels in CNS. aFGF is expressed by a subset of neuronal populations, while bFGF is expressed by astrocytes, both lack signal peptides. Human bFGF is a 17.2kD protein containing 155 amino acid residues. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. Subunit: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2. This protein binds heparin more strongly than does aFGF. Belongs to the heparin-binding growth factors family.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000
Immunohistochemistry: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanSheep
Concentration:
Not Determined
Immunogen:
Recombinant human basic FGF.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile ddH2O.
Specificity:
Recognizes human Basic Fibroblast Growth Factor. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Abraham, et al. (1986) Science. 233(4763):545-8 ; 2. Kurokawa, et al. (1987) FEBS Lett. 213(1):189-94 ; 3. Bieger, et al., (1995) J Neurochem 64, 1521-7 ; 4. Takayama, et al., (1995)Nat Med 1, 53-8. ; 5. Eckenstein (1994) J Neurobiol 25, 1467-80. (1994) ; 6. Abraham, et al., (1986) Embo J 5, 2523-8 ; 7. English, et al., (1995)Dev Biol 169, 57-64.