Technical Data
F4210-15H
Fibroblast Growth Factor, Basic (FGF Basic, FGFb, BFGF, Fibroblast Growth Factor 2, FGF2, Heparin-binding Growth Factor 2, HBGF-2, Prostatropin)
Description:
FGF basic, otherwise known as FGF 2 (fibroblast growth factor 2) is a member of the heparin-binding growth factor family which exists in both cytosolic and nuclear isoforms, ranging in size from 18-24kD, expressed by the majority of cells and tissues. FGF 2 is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumor vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis.

Applications:
Suitable for use in Immunohistochemistry, ELISA, Functional Assays and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Paraffin): 0.25-0.5ug/ml. Requires antigen retrieval using heat treatment prior to staining. Sodium citrate buffer is recommened for this purpose.
ELISA (Indirect or Sandwich): 0.5-2ug/ml
Western Blot: 0.1-0.2ug/ml
Functional Assays: 0.25-0.4ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Pair:
Capture: F4210-15H Fibroblast Growth Factor, Basic
Detection: F4210-15H1 Fibroblast Growth Factor, Basic (Biotin)

Positive Control:
Human breast invasive ductal carcinoma

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant human FGF basic
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes human FGF basic.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Qian, X. et al. (1997) FGF2 concentration regulates the generation of neurons and glia from multipotent cortical stem cells. Neuron. 18: 81-93. 2. Ortega, S. et al. (1998) Neuronal defects and delayed wound healing in mice lacking fibroblast growth factor 2. P.N.A.S. 95: 5672-5677.