Technical Data
F4210-15H
Fibroblast Growth Factor, Basic (FGF Basic, FGFb, BFGF, Fibroblast Growth Factor 2, FGF2, Heparin-binding Growth Factor 2, HBGF-2, Prostatropin)
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Technical Data |
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F4210-15H |
Fibroblast Growth Factor, Basic (FGF Basic, FGFb, BFGF, Fibroblast Growth Factor 2, FGF2, Heparin-binding Growth Factor 2, HBGF-2, Prostatropin) |
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Description: FGF basic, otherwise known as FGF 2 (fibroblast growth factor 2) is a member of the heparin-binding growth factor family which exists in both cytosolic and nuclear isoforms, ranging in size from 18-24kD, expressed by the majority of cells and tissues. FGF 2 is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumor vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis. Applications: Suitable for use in Immunohistochemistry, ELISA, Functional Assays and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (Paraffin): 0.25-0.5ug/ml. Requires antigen retrieval using heat treatment prior to staining. Sodium citrate buffer is recommened for this purpose. ELISA (Indirect or Sandwich): 0.5-2ug/ml Western Blot: 0.1-0.2ug/ml Functional Assays: 0.25-0.4ug/ml Optimal dilutions to be determined by the researcher. Recommended Pair: Capture: F4210-15H Fibroblast Growth Factor, Basic Detection: F4210-15H1 Fibroblast Growth Factor, Basic (Biotin) Positive Control: Human breast invasive ductal carcinoma Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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