Technical Data
F4210-24
Fibroblast Growth Factor 2, Basic, Recombinant, Human (FGF2b)
25ug
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Technical Data |
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F4210-24 |
Fibroblast Growth Factor 2, Basic, Recombinant, Human (FGF2b) |
25ug |
| Growth Factors, Cytokines | Storage: -70°CShipping: Dry Ice |
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FGF-2 is a basic heparin binding growth factor that stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. Biological Activity: Cultures of fetal bovine heart endothelial (FBHE) cells grown for ve days in RD medium containing 2% FBS, 0.1ug/ml heparin, and 2ng/ml of this lot of FGF-2 showed > 12-fold more cells than cultures of FBHE cells grown in same medium without FGF-2. Results may vary depending on cell line used. Cell growth was assessed using an ATP luminescence assay Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months. Molecular Weight: 17.5kD |
Source: Recombinant DNA expressed in yeast (S. cerevisiae). Purity: Purified by heparin-sepharose chromatography. 95% as determined by HPLC and SDS-PAGE. Concentration: ~0.5mg/ml Form: Supplied as a liquid in 0.01mM Tris, pH 7.0, containing 1mM monothioglycerol. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Matuo, Y., et al., In Vitro Cell. Dev. Biol. 24: 477–480, 1988. 2. Matuo, Y., et al., Cytotech. 1: 309–318, 1988.
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