Technical Data
Fibroblast Growth Factor 2, Basic, Recombinant, Human (FGF2b)
Molecular Biology Storage: -70CShipping: Dry Ice
FGF-2 is a basic heparin binding growth factor that stimulates the proliferation of a wide
variety of cells including mesenchymal, neuroectodermal and endothelial cells.

Biological Activity: Cultures of fetal bovine heart endothelial (FBHE) cells grown for ve days in RD medium containing 2% FBS, 0.1ug/ml heparin, and 2ng/ml of this lot of FGF-2 showed > 12-fold more cells than cultures of FBHE cells grown in same medium without FGF-2. Results may vary depending on cell line used. Cell growth was assessed using an ATP luminescence assay

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Molecular Weight:
Source: Recombinant DNA expressed in yeast (S. cerevisiae).

Purity: Purified by heparin-sepharose chromatography. 95% as determined by HPLC and SDS-PAGE.

Concentration: ~0.5mg/ml

Form: Supplied as a liquid in 0.01mM Tris, pH 7.0, containing 1mM monothioglycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Matuo, Y., et al., In Vitro Cell. Dev. Biol. 24: 477480, 1988. 2. Matuo, Y., et al., Cytotech. 1: 309318, 1988.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.