Technical Data
F4210-50A
Fibroblast Growth Factor 4, Recombinant, Human (FGF-4, Heparin Secretory Transforming Protein 1, HSTF-1, HST-1, HST, Transforming Protein KS3, Heparin-binding Growth Factor 4, HBGF-4, HSTF1, KS3)
25ug
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Technical Data |
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F4210-50A |
Fibroblast Growth Factor 4, Recombinant, Human (FGF-4, Heparin Secretory Transforming Protein 1, HSTF-1, HST-1, HST, Transforming Protein KS3, Heparin-binding Growth Factor 4, HBGF-4, HSTF1, KS3) |
25ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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Fibroblast growth factor 4 is a member of the FGF family, a group of 7 homologous polypeptides that act upon a variety of cells and produce biological actions that may include cell proliferation, migration and differentiation. FGF4, also known as oncogene products hst1 and KFGF, has strong transforming potential and is a potent angiogenic factor, being expressed in several highly vascularized tumors. Source: Recombinant corresponding to 182aa from human Fibroblast Growth Factor 4 expressed in E. coli. Molecular Weight: ~19.7kD Endotoxin: 0.1ng/ug Biological Activity: ED50 0.5ng/ml ( 2x10e6 units/mg), determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors. FGF-4 is a heparin binding growth factor expressed by tumor cells and embryonic cells which has mitogenic activity with fibroblasts and endothelial cells. Applications: Suitable for use in Neutralization. Other applications not tested. Recommeded Dilutions: Optimal dilution to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C with a carrier protein. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 19.7kD |
Source: E. coli Purity: ~95% (SDS-PAGE). Purified by sequential chromatography. Concentration: Not determined Form: Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.4, 100mM sodium chloride, 1.25mg BSA. Reconstitute with sterile ddH2O to 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Delli Bovi, P. et al. (1988) Mol. Cell. Biol. 8:2933. 2. Taira, M. et al. (1987) Proc. Nat’l. Acad. Sci. USA 84:2980. 3. Niswander, L. and Martin, G.R. (1992) Development 114:755.55.
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