Technical Data
F4210-50B
FGF-4, Recombinant, Mouse (Fibroblast Growth Factor 4, Heparin-binding Growth Factor 4, HBGF-4, K-fibroblast Growth Factor, Fgf4, Fgf-4, Kfgf)
25ug
Growth Factors, Cytokines Storage: -20CShipping: RT
Fibroblast growth factor-4, also known as FGF-K or K-FGF (Kaposis sarcoma- associated FGF), is a 25kD secreted, heparin-binding member of the FGF family. The mouse FGF-4 cDNA encodes 202aa with a 29aa signal sequence and a 173aa mature protein with an FGF homology domain that contains a heparin binding region near the C-term- inus. Mature mouse FGF-4 shares 87%, 90%, 87% and 85% aa identity with human, rat, canine and bovine FGF-4, respectively. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development. Its expression in the trophoblast inner cell mass pro- motes expression of FGF R2, and is required for maintenance of the troph- ectoderm and primitive endoderm. Later in development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of verte- brate limbs. FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self- renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro. A C-terminally truncated 15kD isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitog- enic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential.

Source:
Recombinant corresponding to aa30-202 of mouse FGF-4, expressed in E.coli (NP_034332).

SDS-PAGE:
19kD, reducing conditions

Activity:
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblasts. The ED50 for this effect is typically 0.75-3.75ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
19kD
Source: E. coli
Purity: ~95% (SDS-PAGE). Endotoxin: 1.0 EU/1ug (LAL).
Concentration: As reported
Form: Supplied as a lyophilized powder from a 0.2um filtered solution in MOPS, ammonium sulfate, EDTA, DTT, BSA. Reconstitute at 100ug/ml in PBS, 0.1% BSA or HSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139. 2. Hebert, J.M. et al. (1990) Dev. Biol. 138:454. 3. Niswander, L. and G.R. Martin (1992) Development 114:755. 4.Feldman, B. et al. (1995) Science 267:246. 5.Goldin, S.N. and V.E. Papaioannou (2003) Genesis 36:40. 6.Sun, X. et al. (2002) Nature 418:501. 7. Boulet, A.M. et al. (2004) Dev. Biol. 273:361. 8. Yu, K and D.M. Ornitz (2008) Development 135:483. 9. Mariani, F.V. et al. (2008) Nature 453:401. 10. Johannesson, M. et al. (2009) PLoS ONE 4:e4794. 11. Kunath, T. et al. (2007) Development 134:2895. 12. Mayshar, Y. et al. (2008) Stem Cells 26:767. 13.Hajitou, A. et al. (1998) Oncogene 17:2059. 14. Flynn, A. and T. O'Brien (2008) IDrugs 11:283.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.