Technical Data
FGF-4, Recombinant, Mouse (Fibroblast Growth Factor 4, Heparin-binding Growth Factor 4, HBGF-4, K-fibroblast Growth Factor, Fgf4, Fgf-4, Kfgf)
Growth Factors, Cytokines Storage: -20CShipping: RT
Fibroblast growth factor-4, also known as FGF-K or K-FGF (Kaposis sarcoma- associated FGF), is a 25kD secreted, heparin-binding member of the FGF family. The mouse FGF-4 cDNA encodes 202aa with a 29aa signal sequence and a 173aa mature protein with an FGF homology domain that contains a heparin binding region near the C-term- inus. Mature mouse FGF-4 shares 87%, 90%, 87% and 85% aa identity with human, rat, canine and bovine FGF-4, respectively. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development. Its expression in the trophoblast inner cell mass pro- motes expression of FGF R2, and is required for maintenance of the troph- ectoderm and primitive endoderm. Later in development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of verte- brate limbs. FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self- renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro. A C-terminally truncated 15kD isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitog- enic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential.

Recombinant corresponding to aa30-202 of mouse FGF-4, expressed in E.coli (NP_034332).

19kD, reducing conditions

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblasts. The ED50 for this effect is typically 0.75-3.75ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: ~95% (SDS-PAGE). Endotoxin: 1.0 EU/1ug (LAL).
Concentration: As reported
Form: Supplied as a lyophilized powder from a 0.2um filtered solution in MOPS, ammonium sulfate, EDTA, DTT, BSA. Reconstitute at 100ug/ml in PBS, 0.1% BSA or HSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.