Technical Data
F4210-50D
FGF4, Recombinant, Human (Fibroblast Growth Factor 4, FGF-4, Heparin Secretory-transforming Protein 1, HST, HST-1, HSTF-1, Heparin-binding Growth Factor 4, HBGF-4, Transforming Protein KS3, HST, HSTF1, KS3)
25ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Fibroblast Growth Factor 4 (FGF-4) is a growth factor predominantly expressed during embryonic development, playing a key role in limb development. In culture, FGF-4 has been shown to be an important growth factor for fibroblasts and endothelial cells. Human FGF-4 shares high homology and cross-reactivity with the mouse protein. Recombinant human FGF-4, produced in E.coli, is a non-glycosylated protein containing 177aa and having a total molecular mass of 19kD.

Source:
Recombinant corresponding to human FGF-4 expressed in E. coli.

AA Sequence:
MAPTAPNGTL EAELERRWES LVALSLARLP VAAQPKEAAV QSGAGDYLLG IKRLRRLYCN VGIGFHLQAL PDGRIGGAHA DTRDSLLELS PVERGVVSIF GVASRFFVAM SSKGKLYGSP FFTDECTFKE ILLPNNYNAY ESYKYPGMFI ALSKNGKTKK GNRVSPTMKV THFLPRL

Molecular Weight:
~19kD

Biological Activity:
Recombinant human FGF-4 has full biological activity when compared standards. The ED50, as determined by its ability to induce the proliferation of mouse NR6R-3T3 fibroblasts, is typically 0.25-1.25ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~19kD
Source: E. coli
Purity: ~97% (SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder from 10mM sodium phosphate, 75mM sodium chloride. Reconstitute with sterile dH2O to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.