Technical Data
Fibroblast Growth Factor 8, Recombinant, Human (FGF8)
Molecular Biology Storage: -20CShipping: RT
Recombinant protein corresponding to human Fibroblost Growth Factor 8 expressed in E. coli.

Endotoxin: <0.1ng/ug

Biological Activity:
ED50 0.5ng/ml ( 2 x 106 units/mg), determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors. FGF-8, also known as Androgen-Induced Growth Factor (AIGF), is a heparin binding growth factor expressed by prespermatogonia in the testes and antral follicles in the ovary.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C with a carrier protein. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: ~95% (SDS-PAGE, HPLC). Endotoxin: 0.1ng/ug
Form: Supplied as a lyophilized powder from 10mM Tris, pH 8.0, 75mM sodium chloride. Reconstitute with 10mM Tris, pH 8.0 to a concentration of 0.1-1.0mg/ml. Do not vortex. Further dilute for extended storage with a buffer containing a carrier protein (0.1% BSA or HSA).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.