Technical Data
F4211-74A
Fibroblast Growth Factor 9, Recombinant, Human (FGF9)
5ug
20ug
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant Human FGF-9 produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 23kDa.
Recombinant FGF-9 is purified by proprietary chromatographic techniques.
The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a single, potential N-linked glycosylation site. The native protein is glycosylated and is efficiently secreted after synthesis, although FGF -9 lacks a typical secretion signal. Rat and mouse FGF-9 show a very high homology to human FGF-9. The transcripts for FGF-9 have been found in brain and in kidney tissue.

Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be
Met-Ala-Pro-Leu-Gly.

Dimers and aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
Recombinant FGF-9 is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5ng/ml, corresponding to a specific activity of 5.5 x 10e6Units/mg.

Endotoxin:
0.1ng/ug (IEU/ug) of Recombinant Human Fibroblast Growth Factor-9.

Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.8 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of FGF-9 as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O or buffer of choice and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
23kD
Source: Sf9 insect cells
Purity: 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Form: Supplied as a lyophilized powder without additives. Reconstitution: It is recommended to reconstitute the lyophilized Recombinant FGF-9 sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.