Technical Data
F4211-75A
Fibroblast Growth Factor 9, Recombinant, Mouse (FGF9)
2ug
10ug
10ug
|
Technical Data |
|
F4211-75A |
Fibroblast Growth Factor 9, Recombinant, Mouse (FGF9) |
2ug 10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
|
Fibroblast Growth Factor-9 (FGF-9, Glial Activating Factor) is a heparin binding growth factor that stimulates the proliferation and activation of glial cells and other cells that express FGF receptors. Murine FGF-9 is a 23.3kD protein containing 205 aa residues. Biological Activity: USBio's murine FGF-9 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is 0.5ng/ml, Corresponding to a specific activity of 2 x 10e6u/mg. Concentration Range for most in vitro applications: 0.1-1ng/ml Responding Cells (partial list): Murine FGF-9 exerts full activity on both murine and human cells (i.e. Glial cells, 3T3 fibroblasts). AA Sequence: PLGEVGSYFG VQDAVPFGNV PVLPVDSPVL LNDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS Storage and Stability: Lyophilized powder may be stored at RT or desiccated at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40mM Tris, pH 8.0, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 23.3kD |
Source: E. coli Purity: 95% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug. Form: Supplied as a lyophilized powder from 20mM Tris, pH 8.0, 75mM sodium chloride. Concentration: 1mg/ml Reconstitution: To obtain a 2 mg/ml solution, add 0.5 µl of water per µg of protein. Dilution into solutions of lower ionic strength should be done immediately before use. For most in vitro applications, FGF-9 exerts its biological activity in the concentration range of 0.1 to 20.0 ng/ml. |
|
1. Chellaiah, A., et al. Mapping Ligand Binding Domains in Chimeric Fibroblast Growth Factor Receptor Molecules. J. Biol. Chem. 274(49): 34785-34794 (1999). 2. Santos-Ocampo, S., et al. Expression and biological Activity of Mouse Fibroblast Growth Factor-9. J. Biol. Chem. and Mol. Bio. Inc. 271(3): 1726-1731 (1996).
|
||