Technical Data
F4212-15B
Fibroblast Growth Factor 12, Recombinant, Human (FGF12, Fibroblast Growth Factor Homologous Factor 1, FHF1, Myocyte Activating Factor, FGF12B)
100ug
500ug
Growth Factors, Cytokines Storage: -70CShipping: Blue Ice
FGF12 (Fibroblast growth factor 12) is a member of the FGF superfamily of molecules which currently stands at 22 members. The FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF12 binds to IB2 (islet brain-2), a cellular kinase scaffold, and voltage gated sodium channels and also plays an important role in intracellular signaling and ion exchange.

Recombinant human FGF12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Source:
Recombinant corresponding to aa1-181 of human FGF12 (His-tagged) expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK
ASLYVAMNGE GYLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ IMKGNRVKKT KPSSHFVPKP
IEVCMYREQS LHEIGEKQGR SRKSSGTPTM NGGKVVNQDS T

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 3ug/ml 15% SDS-PAGE,
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Molecular Weight:
~22.6kD
Source: E. coli
Purity: Purified 90% by SDS-PAGE.
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM tris pH 7.5, 10% glycerol, 1mM DTT, 2mM EDTA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Hubert T., et al. (2008). J Comp Neurol. 507(4):1588-601 2. Nakayama F., et al. (2008). J Radiat Res. 49(5):491-501

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.