Technical Data
F4215-01G
Fibronectin
Description:
The extracellular matrix is a complex network of secreted (glyco)proteins situated in between cells of particular types of tissue. The relative amounts of the different types of matrix macromolecules and the way in which they are organized in the extracellular matrix vary between different tissues. For example, connective tissues have extensive extracellular matrices in which fibrous polymers, especially collagen, are present. In contrast, in epithelial tissues the extracellular matrix consists only of a thin mat called the basal lamina, which underlies the epithelial cell sheet. Cells located in the extracellular matrix produce matrix constituents and control the organization of these matrix components. The main components of the extracellular matrix are proteoglycans (e.g. aggrecan, perlecan and syndecan) and fibrous proteins (e.g. collagen, elastin, fibronectin and laminin). The extracellular matrix, next to being a scaffold to support cells in a particular tissue, also plays a role in the regulation of processes such as proliferation, differentiation, adhesion and locomotion of these cells.

Applications:
Suitable for use in ELISA, Inhibition. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000
Inhibition: 1:100 completely inhibits binding of Staphylococcus aureus to immobilized fibronectin N-terminal domain (coating concentration: 20ug/ml).
Optimum dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgYPurified
SizeStorageShippingSourceHost
10mg-20CBlue IceHumanChicken
Concentration:
~15mg/ml
Immunogen:
N-terminal 29kD fragment of human fibronectin.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity:
Recognizes human fibronectin. Completely inhibits binding of Staphylococcus aureus to immobilized fibronectin N-terminal domain.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.