Technical Data
F4215-02
Fibronectin
Description:
Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml (UV280)
Immunogen:
Fibronectin purified from human plasma.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. No stabilizing proteins added.
Specificity:
Typically less than 1% crossreactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Reacts with human Fibronectin and has negligible crossreactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific crossreactivity of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.