Technical Data
F4215-04A
Fibronectin
Description:
Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG14i21Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
T-cell lymphoma biopsy
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 50% glycerol, 0.1% sodium azide, 0.1% BSA.
Specificity:
Reacts with fibronectin in connectie tissues and vessels. Strongly stains matrix fibronectin in tissues. Animal biodistribution experiments have shown that after intravenous administration, F4215-04A localizes to tumor vessels where it binds to the underlying basement membrane. At the same time, the antigen recognized by F4215-04A is not accessible in normal tissues to circulating antibody indicating that this antibody can be used to target tumor vessels specifically in vivo. Does not recognize a soluble dimeric form of fibronectin (plasma fibronectin). Species Crossreactivity: Human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Epstein, A.L., et al., Cancer Research 55(12): 2673-80 (1995).